Objective To investigate the impact of lidocaine（Lid）on malignant biological behavior of glioma cells by regulating SHH/GLI signal pathway. Methods The U87 cells were divided into U87 group，L⁃Lid group，M⁃Lid group，H⁃Lid group，PM group，and H⁃Lid + PM group. No treatment was performed on U87 cells in L⁃Lid group，M⁃Lid group，and H⁃Lid group. U87 cells were treated with 5，10，and 15 mmol/L Lid，respectively， while PM group was treated with 1 mmol/L Lid μ U87 cells were treated with mol/L of SHH/GLI signal pathway activator PM，while H⁃Lid + PM group was treated with 15 mmol/L Lid and 1 μ The U87 cells were co treated with mol/L of PM. CCK8 was applied to detect U87 cell proliferation；flow cytometry was applied to detect U87 cell apoptosis；Transwell was applied to detect the migration and invasion abilities of U87 cells；Western blot was applied to detect the expression of SHH/GLI protein and apoptosis protein；the tumor⁃bearing mouse model was applied to verify the effect of Lid on U87 cell transplanted tumor. Results Compared with U87 group，the OD value，num⁃ bers of U87 cell migration and invasion，the protein levels of Bcl⁃2，SHH and GLI3，and tumor volume of mice in L⁃Lid group，M⁃Lid group and H⁃Lid group decreased obviously in turn（P < 0.05），the apoptosis rate and Bax protein level increased obviously in turn（P < 0.05）；the OD value，numbers of U87 cell migration and invasion，the protein levels of Bcl⁃2，SHH and GLI3，and tumor volume of mice in PM group were obviously increased（P < 0.05），the apoptosis rate and Bax protein level decreased obviously（P < 0.05）；PM eliminated the inhibitory effect of H⁃Lid on the malignant biological behavior of U87 cells. The survival number of transplanted tumor mice increased after Lid treatment，while the survival number of transplanted tumor mice decreased after PM treatment，and the number in H⁃Lid+PM group was the same as that in L⁃Lid group. Conclusion Lid may inhibit the proliferation of U87 cells， promote cell apoptosis，and then inhibit the progression of glioma by down⁃regulating SHH/GLI signal pathway.